Small angle X-ray scattering on RE31 aptamer

5 Jul 2016, 15:00
1h
2nd and 3rd floors (Budker INP)

2nd and 3rd floors

Budker INP

Board: 033

Speaker

Mr Vladimir Zabluda (Kirensky Institute of Physics Russian Academy of Sciences, Siberian Branch)

Description

This work shows aptamer RE31 (5′-GTGACGTAGGTTGGTGTGGTTGGGGCGTCAC-3′) by small angle X-ray scattering (SAXS) measuring results, wich obtained on DIKSI station in the National Research Center 'Kurchatov Institute'. Also the aptamer structure model is presented, wich was processed in DAMMIN software. According to obtained model, the molecular simulation was constructed with known nucleotides considering interatomic interactions. We have compared aptamer model from SAXS with crystallographic method and confirmed, that SAXS can provide us the information about the size and native shape of bioaptamers in solution. However SAXS method doesn’t require the crystal growing and high concentrations for measuring.

Primary author

Mr Vladimir Zabluda (Kirensky Institute of Physics Russian Academy of Sciences, Siberian Branch)

Co-authors

Dr Alexey Sokolov (Kirensky Institute of Physics SB RAS) Dr Felix Tomilin (Kirensky Institute of Physics SB RAS) Mr Roman Moryachkov (Siberian Federal University, Svobodny 79, 660041, Krasnoyarsk, Russia) Prof. Spiridonova Vera (A.N.Belozersky physico-chemical biology research institute, MSU, Moscow, Russia)

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