Influence of terahertz laser irradiation on the morphology of chicken hepatocytes
- Mr. Eugeny ZELENTSOV
- Mr. Eugeny ZELENTSOV (MC "Integral medicine")
Previously, we have discovered the erythrocyte membrane disruption in aqueous solution under the influence of high-power pulses of the Novosibirsk free electron laser operated at the wavelength of 120-170 microns, with the average power density of 10W/cm2. This fact was explained by the emergence of powerful ultrasound caused by water absorption of terahertz radiation. In this report hepatocytes were studied under the same conditions. The aim of the study was to evaluate the effects of radiation on normal hepatocytes in comparing with previous studies of red blood cells. Liver fragments were sampled and transferred into 500ul of aqueous suspension and irradiated with non-focused beam at different exposure time. Samples were examined histologically using the atomic force microscopy (tapping mode) in the native form. Exposure of 2-4 seconds resulted in single membrane injuries and a slight change in the shape of hepatocytes. Exposure of 5- 10 seconds or more leads to significant damage of hepatocyte membranes. Results have fully confirmed the earlier experiments. Destructive effects of high-power THz pulses on biological objects in the aquatic environment were proven.