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Contribution Poster

Budker INP - 2nd and 3rd floors
X-ray structural analysis

EXPERIENCE OF USE OF X-RAY ENERGY-DISPERSIVE AND MONOCHROMATIC DIFFRACTOMETRY FOR STRUCTURAL RESEARCHES OF BIOLOGICAL SYSTEMS USING SYNCHROTRON RADIATION

Speakers

  • Dr. Vladivir KORNEEV

Primary authors

  • Dr. Vladivir KORNEEV (Institute of Cell Biophysics, RAS, Institutskaya ul. 3, 142290, Pushchino, Russia)

Co-authors

  • Mr. V SHLEKTAREV (Institute of Theoretical and Experimental Biophysics, RAS, Pushchino, Russia)
  • Mr. A ZABELIN (National Research Centre Kurchatov Institute, Moscow, Russia)
  • Dr. N LANINA (Institute of Theoretical and Experimental Biophysics, RAS, Pushchino, Russia)
  • Dr. B TOLOCHKO (Budker Institute of Nuclear Physics, SB, RAS, Novosibirsk, Russia)
  • Dr. A VAZINA (Institute of Theoretical and Experimental Biophysics, RAS, Pushchino, Russia)

Content

The important role in the study of the structural mechanism of the functional activity of biological systems belongs to the instrumental and methodical developments intended for the energy-dispersive (Θ=const) and monochromatic (λ=const) diffractometry with synchrotron radiation (SR) usage. Results of our long-term developments are considered. At one method the collimated X-ray beam of polychromatic radiation ("white spectrum") is directed on the object, and the diffraction pattern represent the intensity distribution as function of a wavelength at the fixed scattering angle (Θ), i.e. lattice-plane spacing of object are uniquely determined by a discrete set of the photon energy. Note the spatial resolution of different zones of the X-ray pattern can be changed by varying Θ value that allowed to record weak reflections and to register X-ray pattern with high resolution for material with large spacings of identity. The ‘λ=const’ method determines by dedicated monochromatic wavelength from continuous SR spectrum, and then the X-ray beam is collimated by X-ray optical zoom lenses; obtained diffraction patterns demonstrate the intensity distribution as a function of scattering angle. The ‘Θ=const’ method provides increasing intensity of the X-ray beam on 2-3 orders, however earlier during creation of the first SR sources these advantages were unattainable because of under quick-action of the detection system. The ‘λ=const’ method is traditionally used with position sensitive coordinate detectors. Now power-dispersion detectors with high performance of registration and energy resolution are developed, therefore it is very inviting to use polychromatic radiation. Various X-ray diffraction stations created by us (KEMUS, FRAKS, DICSI) on the operating channels of the storage rings VEPP-3, VEPP-4 (Siberian Center of Synchrotron and Terahertz Radiations, SCSTR, Novosibirsk) and Siberia-2 (Kurchatov Center of Converging of Nano-, Bio-, Information-, and Cognitive Sciences and Technologies, NBIC, Moscow) are presented. This equipment based on block - modular configuration, i.e. the main devices of stations could be used as independent modules for any experimental scheme. Despite different character of current tasks, a many things in the experimental technique were unified, for example, such blocks as monochromatic and reflecting zoom lenses, collimators of the primary beam, and also the vacuuming device, remote control of the optical system components and software. Results of the SAXS/WAXS diffraction investigations of biological tissues at different physiological states are considered. There are presented also the first experimental results of X-ray diffraction study of test objects (collagen and muscle) received by the Θ=const method with using SR of the VEPP-3 storage ring. The work is supported by the RFBR grant # 14-44-03667.