Home > Timetable > Session details > Contribution details

Contribution Poster

Budker INP - 2nd and 3rd floors

Synergistic influence of HF welding on nanostructiral orderliness of epithelial tissues of gastrointestinal tract

Speakers

  • Dr. Alvina VAZINA

Primary authors

Co-authors

  • Anna VASILIEVA (Institute of theoretical and experimental biophysics, RAS, Pushchino, Russia)
  • Michail KVASHA (Romodanov’ Institute of Neurosurgery, Kiev, Ukraine)
  • Nadezhda LANINA (Institute of theoretical and experimental biophysics, RAS, Pushchino, Russia)
  • Aleksander ZABELIN (NRC "Kurchatov Institute", Moscow, Russia)
  • Dr. Vladivir KORNEEV (Institute of Cell Biophysics, RAS, Institutskaya ul. 3, 142290, Pushchino, Russia)
  • Victor VASILIEV (Institute of protein, RAS, Pushchino, Russia)
  • Georgiy MARINSKY (Paton’ Electric Welding Institute, NASU, Kiev, Ukraine)
  • Sergey S. PODPRYATOV (Municipal Clinical Hospital No. 1; Paton’ Electric Welding Institute, NASU, Kiev, Ukraine)
  • Sergey E. PODPRYATOV (Municipal Clinical Hospital No. 1; Paton’ Electric Welding Institute, NASU, Kiev, Ukraine)
  • Sergey GICHKA (Municipal Clinical Hospital No. 1, Kiev, Ukraine)

Content

The results of study of influence of high-frequency electrosurgical welding (HF welding) on molecular and nanostructiral orderliness of thin intestine tissues of a pig - mucosa, submucosa, muscle layers, serosa, and also the purify mucin preparations from pig’s gastrointestinal tract are presented. Structural study of biological tissues by X-ray diffraction methods with synchrotron radiation usage were carried out on constructed by us small-angle station «diffraction cinema» DICSI at the NRC «Kurchatov institute», Moscow. Considerable distinctions on X-ray patterns of thin intestine tissues are registered before and after HF welding: the X-ray patterns of native tissue display a small-angle diffuse scattering; diffraction lines practically do not reveal. After application of HF welding, the small-angle X-ray patterns of welded sutures in intestinal tissue are literally filled with sharp diffraction rings: a series of sharp Debye rings with main spacing of 4.65 nm was recorded within the nanoscale range of 10 to 0.4 nm. We earlier attributed the nanostructural invariant of 4.65(±0.15) nm as periodicity caused by regular attachment of oligosaccharide chains to the protein core of giant proteoglycan molecule of mucus and extracellular matrix of tissues [A. Vazina et al., Bullet. RAS, 2013, 77(2)]. Considerable changes in X-ray patterns of gastrointestinal tissues under synergistic influence of various parametres of welding (temperature, toolkit geometry, pressure, modulation of an electromagnetic field, etc.) can be interpreted by increase in concentration of scattering components due to cold steam formation at acoustic excitation of a site of a tissue between electrodes. Thus, under the influence of welding in tissues it is formed high ordered proteoglycan scaffold providing the functional arrangement of cells during reparation. Proteoglycan systems can be not only a marker of the physiological status of a tissue, but also a regulator providing adequate adaptation of biological systems to change of external parametres of environment. The research was supported by RFBR-Moscow region Grant #14-44-03667.