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SUMMARY:Small-Angle scattering applications to the analysis of aptamer str
 ucture and conformational changes
DTSTART;VALUE=DATE-TIME:20200714T113000Z
DTEND;VALUE=DATE-TIME:20200714T115000Z
DTSTAMP;VALUE=DATE-TIME:20260717T175214Z
UID:indico-contribution-1832@indico.inp.nsk.su
DESCRIPTION:Speakers: Roman Moryachkov (Kirensky Institute of Physics)\nAt
  present different agents are developing for diagnostics and treatment of 
 cancer - one of the main reasons of human deaths. Especially perspective e
 xamples are aptamers - single-stranded DNA or RNA molecules which are comp
 act analogs of the antibodies with low immune response\, have a short leng
 th (10-100 nucleotides)\, well-defined spatial structure and surface charg
 e distribution providing highly specific binding with their targets - prot
 eins in the tumor cells. It allows one to use aptamers as the agents for t
 he visualisation the cancer cells and tissues in organism and also as the 
 carriers for active molecules\, labels\, nanoparticles\, toxic entities to
  deseased tissue cells. To define the function and parameters of the activ
 ity for the aptamers and to localize the specific binding epitopes on the 
 surface of both aptamers and the target proteins\, it is necessary to know
  the spatial structure of these molecules.\nFor this purpose the Small-Ang
 le X-ray Scattering (SAXS) method was used\, which is applied in a high qu
 ality at the synchrotron radiation sources due to the high intensity of th
 e emitted X-rays\, ability to change the wavelength and beam size for the 
 experiment. SAXS method is used to carry out the measurements on the biomo
 lecules in solution\, without crystallisation which is mostly impossible f
 or single-stranded nucleic acids. One can create a native environment for 
 the molecules under study\, or inversely to track the conformational chang
 es during varying temperature\, pH and other parameters.\nHere we present 
 the results of revealing and adjusting the 3D spatial structures of the DN
 A molecules using the SAXS method in solution and the molecular simulation
  method based on the experimental SAXS data. This combination yields an in
 formation about the molecule structure\, possible conformational changes\,
  parameters of the aptamer-protein complexation\, that in a perspective wi
 ll be useful to optimize the development procedure of the theranostics age
 nts against the socially important deseases.\n\nThis work is partially sup
 ported by RFBR grant №18-32-00478.\n\nhttps://indico.inp.nsk.su/event/24
 /contributions/1832/
LOCATION: Zoom 890 9721 5207
URL:https://indico.inp.nsk.su/event/24/contributions/1832/
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