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SUMMARY:The study of the nonthermal effects of terahertz radiation on livi
 ng systems.
DTSTART;VALUE=DATE-TIME:20160705T044000Z
DTEND;VALUE=DATE-TIME:20160705T050000Z
DTSTAMP;VALUE=DATE-TIME:20260513T170029Z
UID:indico-contribution-1169@indico.inp.nsk.su
DESCRIPTION:Speakers: Sergey Peltek (Institute of Cytology and Genetics SB
  RAS (Novosibirsk\, Russia))\nMescheryakova I.A. 1\, Demidova E.V. 1\,  Go
 ryachkovskaya T.N. 1\, Demidov E.A. 1\, Bryanskaya A.V. 1\, Sergeeva S.V. 
 1\, Kiselev S.L. 3\, Lagarkova M.A. 3\,  Kulipanov G.N. 2\, Semenov A.I.2\
 , Vinokurov N.A.2\, Kolchanov N.A.1\, Popik V.M.2\, Peltek S.E.1\n1 The In
 stitute of Cytology and Genetics The Siberian Branch of the Russian Academ
 y of Sciences\; 2 Budker Institute of Nuclear Physics the Siberian Branch 
 of the Russian Academy of Sciences\; 3 Vavilov Institute of General Geneti
 cs\, RAS\, Moscow\, \n\nIn connection with the increasingly widespread use
  of terahertz radiation in the practice and the lack of consensus on the b
 iological effects of terahertz radiation\, it seems urgent to study in det
 ail the effects of such exposure as model organisms\, including bacteria a
 nd human cells.\n\nWe studied the impact of terahertz radiation on E. coli
  biosensor cells containing plasmids with promoters of stress-sensitive ge
 nes controlling the expression of GFP. GFP level was measured by fluoromet
 ry. The impact of terahertz radiation was nonthermal\, i.e. special care w
 as taken to keep specimen temperature in the 35±2 °С range during irrad
 iation so that heat shock genes would not be induced. GFP level was measur
 ed by fluorometry.\n\nWe found that terahertz radiation activates genes as
 sociated with oxidative stress response. Results of Ames test and SOS-chro
 motest indicate that terahertz radiation doses tested no direct action on 
 DNA mutagenic and does not genotoxic.\n\nProteomic analysis\, it was found
  that exposure to E. coli cells terahertz radiation leads to increased exp
 ression of 14 genes of rapid response. Among these genes discovered glutam
 ine synthetase gene (glnA). Based glnA gene promoter designed biosensor se
 nsitive to the effects of terahertz radiation.\nHuman embryonic stem cells
  (hESCs) are extremely sensitive to environmental stimuli\, and we therefo
 re utilised this cell model to investigate the non-thermal effects of THz 
 irradiation. We studied DNA damage and transcriptome responses in hESCs ex
 posed to narrow-band THz radiation (2.3 THz) under strict temperature cont
 rol.\n\nhttps://indico.inp.nsk.su/event/3/contributions/1169/
LOCATION:Budker INP Conference Hall
URL:https://indico.inp.nsk.su/event/3/contributions/1169/
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