BEGIN:VCALENDAR VERSION:2.0 PRODID:-//CERN//INDICO//EN BEGIN:VEVENT SUMMARY:Biomedical Applications of Terahertz Radiation DTSTART;VALUE=DATE-TIME:20160705T042000Z DTEND;VALUE=DATE-TIME:20160705T044000Z DTSTAMP;VALUE=DATE-TIME:20260712T180232Z UID:indico-contribution-139-1156@indico.inp.nsk.su DESCRIPTION:Speakers: Vincent Wallace (UWA)\nEarly experiments by pioneers of THz imaging demonstrated that the technique could have a variety of ap plications\, including medicine and dentistry - terahertz images of biolog ical tissues showed contrast between lean meat and fat\; changes in refrac tive index of teeth at THz frequencies allow for the detection of early de cay. \n\nSince then there have been many studies of tissues\, from wax em bedded tissue block\, histopathological samples\, excised tissues and even some in vivo imaging. Some of this most significant work revealed contras t between regions of healthy skin and basal cell carcinoma\, the most comm on form of skin cancer\, in vitro and in vivo. Further\, other work on exc ised breast carcinoma has shown good contrast between tumours and normal t issues. This has led to the development of a prototype handheld (intra-ope rative THz) imaging probe for use during breast surgery. \n\nThe high abso rption of THz by water in this range (23 mm−1 at 1 THz) makes transmissi on imaging through a body impossible\, but it is the difference in absorpt ion due to water content which explains the contrast seen between muscle a nd adipose tissue and between tumour and normal tissue. Terahertz spectros copic measurements of carcinoma show significant differences in the freque ncy dependent refractive index and absorption coefficient. Such contrast o n the surface is often obvious at optical frequencies but it is the abilit y of THz to penetrate below the surface that provides the potential for me dical imaging\, in particular\, of epithelial tissues (cancer with origins in tissue surfaces both external and internal)\, which includes skin\, br east and colon cancer\, accounts for 85% of all cancers. \n\nIt is the abi lity of THz to penetrate just below the surface that provides the\, as yet not fully realised\, potential for tissue imaging\, visualisation of the subsurface spread of tumours and other applications. Full understanding of the contrast mechanisms in tissues and their physiological significance w ill enable THz to fulfil its potential for medical applications.\n\nTo dat e in vivo measurements have been limited due to the restricted nature of i maging systems. Compact\, mobile THz imaging systems are now being develop ed which allows for TPI measurements in a clinical setting.\n\nhttps://ind ico.inp.nsk.su/event/3/contributions/1156/ LOCATION:Budker INP Conference Hall URL:https://indico.inp.nsk.su/event/3/contributions/1156/ END:VEVENT BEGIN:VEVENT SUMMARY:The study of the nonthermal effects of terahertz radiation on livi ng systems. DTSTART;VALUE=DATE-TIME:20160705T044000Z DTEND;VALUE=DATE-TIME:20160705T050000Z DTSTAMP;VALUE=DATE-TIME:20260712T180232Z UID:indico-contribution-139-1169@indico.inp.nsk.su DESCRIPTION:Speakers: Sergey Peltek (Institute of Cytology and Genetics SB RAS (Novosibirsk\, Russia))\nMescheryakova I.A. 1\, Demidova E.V. 1\, Go ryachkovskaya T.N. 1\, Demidov E.A. 1\, Bryanskaya A.V. 1\, Sergeeva S.V. 1\, Kiselev S.L. 3\, Lagarkova M.A. 3\, Kulipanov G.N. 2\, Semenov A.I.2\ , Vinokurov N.A.2\, Kolchanov N.A.1\, Popik V.M.2\, Peltek S.E.1\n1 The In stitute of Cytology and Genetics The Siberian Branch of the Russian Academ y of Sciences\; 2 Budker Institute of Nuclear Physics the Siberian Branch of the Russian Academy of Sciences\; 3 Vavilov Institute of General Geneti cs\, RAS\, Moscow\, \n\nIn connection with the increasingly widespread use of terahertz radiation in the practice and the lack of consensus on the b iological effects of terahertz radiation\, it seems urgent to study in det ail the effects of such exposure as model organisms\, including bacteria a nd human cells.\n\nWe studied the impact of terahertz radiation on E. coli biosensor cells containing plasmids with promoters of stress-sensitive ge nes controlling the expression of GFP. GFP level was measured by fluoromet ry. The impact of terahertz radiation was nonthermal\, i.e. special care w as taken to keep specimen temperature in the 35±2 °С range during irrad iation so that heat shock genes would not be induced. GFP level was measur ed by fluorometry.\n\nWe found that terahertz radiation activates genes as sociated with oxidative stress response. Results of Ames test and SOS-chro motest indicate that terahertz radiation doses tested no direct action on DNA mutagenic and does not genotoxic.\n\nProteomic analysis\, it was found that exposure to E. coli cells terahertz radiation leads to increased exp ression of 14 genes of rapid response. Among these genes discovered glutam ine synthetase gene (glnA). Based glnA gene promoter designed biosensor se nsitive to the effects of terahertz radiation.\nHuman embryonic stem cells (hESCs) are extremely sensitive to environmental stimuli\, and we therefo re utilised this cell model to investigate the non-thermal effects of THz irradiation. We studied DNA damage and transcriptome responses in hESCs ex posed to narrow-band THz radiation (2.3 THz) under strict temperature cont rol.\n\nhttps://indico.inp.nsk.su/event/3/contributions/1169/ LOCATION:Budker INP Conference Hall URL:https://indico.inp.nsk.su/event/3/contributions/1169/ END:VEVENT BEGIN:VEVENT SUMMARY:Synchrotron Small-Angle X-Ray Scattering as Universal Instrument o f Structural Analysis of Bio and Nanosystems DTSTART;VALUE=DATE-TIME:20160705T074000Z DTEND;VALUE=DATE-TIME:20160705T080000Z DTSTAMP;VALUE=DATE-TIME:20260712T180232Z UID:indico-contribution-139-1212@indico.inp.nsk.su DESCRIPTION:Speakers: Eleonora Shtykova (Shubnikov Institute of Crystallog raphy of Federal Scientific Research Centre “Crystallography and Photoni cs” of Russian Academy of Sciences)\nSmall-angle X-ray scattering is a u niversal diffraction method for studying the supra-atomic structure of mat ter. The potential of this technique has greatly increased in recent years due to the development of bright synchrotron radiation sources. The exten sive use of these sources\, in combination with new techniques for interpr etation of scattering data and structural modeling\, made small-angle scat tering one of the most effective analytical methods to study nanoscale str uctures. In the present work\, after a brief outline of the basic principl es of small-angle scattering by isotropic dispersed nanosystems\, two area s of nanodiagnostics\, in which the progress in the small-angle experiment and the latest techniques for interpreting scattering data has become pro nounced in recent years\, will be demonstrated. These areas - the analysis of the structure of biological macromolecules in a solution and structura l studies of metal nanoparticles synthesized in polymer and aqueous media - are illustrated by examples of practical biological and nanotechnologyca l applications.\n\nThis work was supported in part by Russian Foundation f or Basic Researches (projects 15-54-74002 EMBL\, 16-03-00375\, 16-03-00379 and 16-29-11765).\n\nhttps://indico.inp.nsk.su/event/3/contributions/1212 / LOCATION:Budker INP Conference Hall URL:https://indico.inp.nsk.su/event/3/contributions/1212/ END:VEVENT BEGIN:VEVENT SUMMARY:Analysis of K-lines X-ray fluorescence of Rare-Earth and High-Z el ements on storage ring of the VEPP-4M DTSTART;VALUE=DATE-TIME:20160705T082000Z DTEND;VALUE=DATE-TIME:20160705T084000Z DTSTAMP;VALUE=DATE-TIME:20260712T180232Z UID:indico-contribution-139-1222@indico.inp.nsk.su DESCRIPTION:Speakers: Aleksandr Legkodymov (Alekseevich)\nX-ray fluorescen ce analysis with high-energy photons (40–100 keV) is described. Measurem ent of K-lines from high-Z elements has attractive advantages\, which are not available by L-line analysis. The experimental data on the minimum det ection limits (MDLs) of rare-earth elements from La to Lu in standards of igneous rocks are described. The first results were obtained at the minimu m detection limits of heavy platinoids (Os\, Ir\, Pt\, Au) based on the li nes of the K series of the characteristic radiation excited by photons wit h energy 100 keV.\nThe main parameters of the beamline\, layout\, monochro matization system and detection system of the X-ray fluorescent are descri bed.\n\nhttps://indico.inp.nsk.su/event/3/contributions/1222/ LOCATION:Budker INP Conference Hall URL:https://indico.inp.nsk.su/event/3/contributions/1222/ END:VEVENT BEGIN:VEVENT SUMMARY:Study of the THz response of protein solutions at different stages of glycation DTSTART;VALUE=DATE-TIME:20160705T070000Z DTEND;VALUE=DATE-TIME:20160705T072000Z DTSTAMP;VALUE=DATE-TIME:20260712T180232Z UID:indico-contribution-139-1229@indico.inp.nsk.su DESCRIPTION:Speakers: Olga Cherkasova (Institute of Laser Physics of SB RA S)\nProtein glycation is accelerated under hyperglycemic conditions result ing in loss of the structure and biological functions of proteins. This pr ocess plays a significant role in diabetic complications in organism tissu es. THz spectroscopy is unique tool that can be used for analysis of biolo gical tissues and solutions because changes in relative proportions of fre e and bound water and in relaxation times for either of these states can a ll be observed in THz range. A distinctive feature of this method is the p ossibility of measuring directly the refractive index\, absorption coeffic ient\, and hence complex permittivity spectrum of the sample in a single s can and in a broad frequency range.\n The THz time-domain spectroscopy ha s been used for measuring of bovine serum albumin (BSA) and monosaccharide s solutions and studying of glycation dynamics for 0-96 h incubation BSA w ith sugars. To detect small-scale changes in solutions and to increase the significance of observations\, we have performed measurements using both transmission and attenuated total internal reflection (ATR). By combining the results obtained in both configurations\, the reliable range of the sp ectrum can be considerably broadened (0.05-2.5 THz). \n The THz time-doma in spectrometer used in the study was described previously [1]. The Fourie r transform of the measured pulse time profile provides the complex spectr um comprising information on the refractive and absorption indices of the medium where the pulse has been reflected or transmitted. To recalculate t he transmission spectrum into the absorption and refraction spectra\, we u sed the Fresnel formulas and the methods described in [2].\n We analyzed the reasons for the THz transmission changes of BSA and monosaccharides so lution during incubation. The differences were analyzed by comparing exper imental spectra to the model dielectric function of water. THz absorption coefficient of glycated albumin solution increases during first several da ys of incubation. Fructose demonstrated stronger and/or faster influence o n THz absorption of BSA than glucose. We consider that glycation of BSA re sults in a change the parameters of slow Debye relaxation. These results s how that THz spectroscopy is a useful tool for study of protein glycation in time.\n\nREFERENCES\n\n[1] Nazarov\, M. M.\, Shkurinov\, A. P.\, Kulesh ov\, E. A.\, Tuchin\, V. V.\, “Terahertz time-domain spectroscopy of bio logical tissues”\, Quantum Electron. 38\, 647-654 (2008).\n[2] Cherkasov a\, O.P.\, Nazarov\, M. M.\, Angeluts\, A.A.\, Shkurinov\, A.P.\, “The I nvestigation of blood plasma in the terahertz frequency range”\, Optics and Spectroscopy 120(1)\, 50-57(2016).\n\ncorresponding author:\nCherkasov a Olga Pavlovna\,\n630090\, Novosibirsk\, Prospect ak. Lavrent'ev\, 13/3\; \nE-mail: o.p.cherkasova@gmail.com Tel: (383) 330-99-22\, 8-965-823-11-92\ n\nhttps://indico.inp.nsk.su/event/3/contributions/1229/ LOCATION:Budker INP Conference Hall URL:https://indico.inp.nsk.su/event/3/contributions/1229/ END:VEVENT BEGIN:VEVENT SUMMARY:Radiation therapy of human glioma tumors experiments in SSTRC DTSTART;VALUE=DATE-TIME:20160705T050000Z DTEND;VALUE=DATE-TIME:20160705T052000Z DTSTAMP;VALUE=DATE-TIME:20260712T180232Z UID:indico-contribution-139-1236@indico.inp.nsk.su DESCRIPTION:Speakers: Konstantin Kuper (Budker Institute of Nuclear Physic s)\nIn modern society\, brain diseases belong to most frequent causes of d eath and reduction of the working age. For prevention and treatment of neu ropathology\, a variety of therapeutic and surgical approaches are being d eveloped and improved\, including radiation therapy methods. These methods have got a significant impetus to improvement through the creation of hig h-energy radiation sources and development of tools providing accurate tar geting and strict time dosing in radiotherapy. Microbeam synchrotron radia tion is one of the most promising trends in the modern radiotherapy. This technology is based on the use of synchrotron flux split into separate mic robeams. Such spatial arrangement allows one to significantly amplify the dose on targets\, e.g. tumors\, without causing significant damage to heal thy tissue.\nIn our work we try to offer new possibilities for improving t herapeutic technologies based microbeam radiation which is consists in com bined use of radiotherapy and applying nanoparticles. In our experiments w e used of combination of the effect of radiotherapy with saturation of tum or cell with nanoparticles of metal oxides\, including particles with high catalytic activity. This combination may provide a synergistic effect fo r cytolysis of glioma cells based on nanoparticles enhance oxidative effe ct of reactive oxygen.\nIn present time we search optimal combination of c omplex approach to the radio and chemo therapy of human glioma tumors.\n\n https://indico.inp.nsk.su/event/3/contributions/1236/ LOCATION:Budker INP Conference Hall URL:https://indico.inp.nsk.su/event/3/contributions/1236/ END:VEVENT BEGIN:VEVENT SUMMARY:Structural parameters of macroscopically flat lipid multilayers on a silica sol substrates DTSTART;VALUE=DATE-TIME:20160705T072000Z DTEND;VALUE=DATE-TIME:20160705T074000Z DTSTAMP;VALUE=DATE-TIME:20260712T180232Z UID:indico-contribution-139-1243@indico.inp.nsk.su DESCRIPTION:Speakers: Yuriy Volkov (Shubnikov Institute of Crystallography \, FSRC “Crystallography and Photonics” RAS)\nA phospholipid bilayer o n liquid substrate can be used as the simplest model of a cell membrane [1 ]. Previously\, the method of preparation of macroscopically flat regular lipid multilayers with the use of colloidal silica hydrosols as substrates has been proposed [2]. Due to the specific boundary conditions at air/sol interface\, polar lipid molecules exhibit a spontaneous ordering effect.\ n\nIn this report we present the investigations of influence of hydrosol c ompound (i.e. size of silica nanoparticles\, pH level and concentration of alkali ions) on the structural parameters of phospholipid layers by the X -ray reflectometry method with the use of synchrotron radiation. Model pho sphatidylcholine lipids DSPC\, DPPC and SOPC have been used as samples. Ex periments have been carried at X19C beamline (radiation energy E = 15 keV) of NSLS facility\, Brookhaven. Depth-graded distributions of electron den sity have been extracted from the measured reflectivity curves by the mode l-independent reconstruction algorithm [3].\n\nAccording to the obtained r esults\, overall thickness of lipid film is consistent with the Debye leng th for the substrate sol and thus can be varied from the monolayer (d ~ 35 Å) to the stack of bilayers (d ~ 450 Å) at pH $\\approx$ 11.5 and 9 res pectively. Separate mono- and bilayers exhibit high degree of structural p erfection\, with calculated lipid area $A = 45 \\pm 2$ and $49 \\pm 3$ Å$ ^2$ being in agreement with the theoretical values for 2-dimensional phosp holipid crystal. Enrichment of the substrate with Na$^+$ and Cs$^+$ leads to the absorption of alkali ions into the lipid membrane\, with the surfac e concentration of ions up to $2 \\times 10^{19}$ m$^{-2}$.\n\n[1] D. M. S mall\, "The Physical Chemistry of Lipids"\, Plenum Press\, NY (1986).\n\n[ 2] A. M. Tikhonov\, JETP Letters\, 2010\, 92:394.\n\n[3] I. V. Kozhevnikov \, Nucl. Instr. Meth. Phys. Res. A\, 2003\, 508:519.\n\nhttps://indico.inp .nsk.su/event/3/contributions/1243/ LOCATION:Budker INP Conference Hall URL:https://indico.inp.nsk.su/event/3/contributions/1243/ END:VEVENT BEGIN:VEVENT SUMMARY:Investigation of the principles of detection of small amounts of n anoparticles of drugs and their conglomerates during the synthesis and tra nsport in vivo using methods of X-ray synchrotron radiation DTSTART;VALUE=DATE-TIME:20160705T054000Z DTEND;VALUE=DATE-TIME:20160705T060000Z DTSTAMP;VALUE=DATE-TIME:20260712T180232Z UID:indico-contribution-139-1250@indico.inp.nsk.su DESCRIPTION:Speakers: Alexey Ancharov (Institute of Solid State Chemistry and Mechanochemistry SB RAS)\nThe basic idea of the investigation is using the unique properties of the synchrotron radiation can receive informatio n about the drug are in the gastrointestinal tract (GIT) of a living body at low concentrations. In particular: the phase state\, i.e\, tablet disso lved in the stomach or not\; the charge state\, i.e\, the drug is active o r passive\; of the time (and place) of sedimentation of the drug\; about t he localization of the drug on the damaged wall of the digestive tract.\nI n this project\, the first steps was made towards the realization of the i dea with using a model system. The experiment was conducted at the experim ental station of synchrotron radiation (SR) №4 VEPP -3. Medicament (in o ne experiment in the crystal\, and in the other - in liquid form) was pour ed into the stomach of a turkey\, and irradiated with a narrow beam with a n energy 33.7 keV. With area detector mar-350 diffraction pattern of the d rug was reseved\, imposed on the scattering from the tissue of the stomach . The main objective was to allocate the sum of the diffraction pattern in formation about the structure of the drug. The main result of the experime nt - using SR can receive information about the crystal lattice of drugs i n real biological.\nThus\, using the an unique properties of synchrotron r adiation in this study managed to obtain structural information about drag \, are in conditions close to real ones (in terms of interaction with X-ra ys) - virtually the same as in the stomach of a living organism. The drug was in solid or in dissolved form.\nAs an object of research we selected a nti-ulcer drug substance based on bismuth tripotassium dicitrate (BTD). BT D is a drug with bactericidal activity against Helicobacter pylori has ant i-inflammatory and astringent. Work was carried out on behalf of the Minis try of Health RF for the development of domestic analogue drugs De Nol (Ne therlands).\nThe project succeeded in synthesizing a complete analogue of imported medicines De-Nol (Netherlands) and to explore its metabolism in t he chemical environment similar to the environment in different parts of t he gastrointestinal tract.\nBy simulating the behavior of BTD in the gastr ointestinal tract by small-angle x-ray scattering discovered that bismuth forms a stable complex of a colloidal particle. The particle has size in o rder of 22 A. If the solution is diluted with water particle size changes. \nThe structure of the colloidal particles was investigated by X-ray diffr action on a synchrotron radiation beam. It was found that the structure is sensitive to pH\n\nhttps://indico.inp.nsk.su/event/3/contributions/1250/ LOCATION:Budker INP Conference Hall URL:https://indico.inp.nsk.su/event/3/contributions/1250/ END:VEVENT BEGIN:VEVENT SUMMARY:Nanoradiator therapy and synchrotron X-ray imaging of malignant br ain tumor DTSTART;VALUE=DATE-TIME:20160705T052000Z DTEND;VALUE=DATE-TIME:20160705T054000Z DTSTAMP;VALUE=DATE-TIME:20260712T180232Z UID:indico-contribution-139-1293@indico.inp.nsk.su DESCRIPTION:Speakers: Jong-Ki Kim (Catholic University of Daegu\, School o f Medicine)\nGlioblastoma malformation is typical intractable malignant br ain tumor that has less than 2 years as mean survival due to infiltrative nature in conjunction with microvascular malformation (MVP). Conventional drug delivery is limited by intact BBB in normal tissue surrounding MVP\, and current radiotherapy is not indicative due to infiltration of tumor c ell into normal brain. Therefore\, tumor-infiltrating MVP should be therap eutic target. Recently we imaged MVP with tumor infiltration in rat glioma model using synchrotron X-ray DEI CT technique. High-Z nanoparticles play a role as nanoradiator\, burst release of low energy electrons and charac teristic X-ray fluorescence\, when subject to X-ray photoelectric ionizati on or high-energy ion beam-irradiated Coulomb scattering. During last deca de we have developed dosimetry of nanoradiator effect and medical applicat ion of novel site-specific nanoradiator therapy using either monochromatic synchrotron X-ray or traversing Bragg-peak proton beam. Eventually nanora diator therapy provide a new platform of therapeutic nanobeacon technology by converting prodrug inert gold/iron nanoparticle into electron-emitting drug. Realization of this concept in oncology and non-oncology would be d emonstrated in this presentation. In addition\, high-resolution X-ray diff raction CT imaging of skull-based brain is challenging and discussed with our resolving efforts\n\nhttps://indico.inp.nsk.su/event/3/contributions/1 293/ LOCATION:Budker INP Conference Hall URL:https://indico.inp.nsk.su/event/3/contributions/1293/ END:VEVENT BEGIN:VEVENT SUMMARY:The possibility of direct analysis of biological tissues of a few milligrams by SR XRF method DTSTART;VALUE=DATE-TIME:20160705T080000Z DTEND;VALUE=DATE-TIME:20160705T082000Z DTSTAMP;VALUE=DATE-TIME:20260712T180232Z UID:indico-contribution-139-1305@indico.inp.nsk.su DESCRIPTION:Speakers: Valentina Trunova (Nikolaev Institute of Inorganic C hemistry SB RAS)\nDevelopment and improvement of analytical methods to exp lore the element and microelement composition of biological tissues enable s obtaining additional information on functioning of systems of organs and tissues. It is the information on the multi-element composition of biopsy material of living organisms that is relevant. Since this material always has very low weight\, 4 mg to 0.5 mg of dry weight\, such amount of sampl e material cannot be directly explored by the conventional non-destructive methods of analysis. The works performed use the unique properties of syn chrotron radiation\, which enable analysis of small-weight samples (a few milligrams)\, which is virtually impossible with the conventional methods. The SR XRF method enables analysis of small-weight samples and selection of optimal conditions for analysis of the elements. For nine years\, the e xperimental station for X-ray fluorescence analysis (VEPP-3\, BINP) has be en involved in joint systematic exploration with Meshalkin Research Instit ute of Blood Circulation Pathology. About 1000 samples of the vascular sys tem in patients with different pathologies have been analyzed: congenital heart disease\, coronary heart disease\, dilated cardiomyopathy (heart tra nsplant)\, aortic aneurysm\, etc. A new sample-preparation method for dire ct analysis of biopsy material weighing to 0.5 mg was developed. A techniq ue of analysis of fragments of biopsy and surgical samples by K-lines of S \, Cl\, K\, Ca\, Sc\, Ti\, V\, Cr\, Mn\, Fe\, Co\, Ni\, Cu\, Zn\, As\, Se\ , Br\, Kr \, Rb\, Sr\, and Mo was developed. The results of the determinat ion of the elemental composition of fragments of biopsy and autopsy sample s of cardiovascular disease by the SR XRF method gave a method of evaluati ng the functional state of the heart. It can be used in clinical practice for pre-testing in pathology of heart transplant and in aortic dissection. \n\nhttps://indico.inp.nsk.su/event/3/contributions/1305/ LOCATION:Budker INP Conference Hall URL:https://indico.inp.nsk.su/event/3/contributions/1305/ END:VEVENT END:VCALENDAR