Speaker
Dr
Yury Gaponov
(Department of atmospheric research, Novosibirsk State University, Novosibirsk, Russia)
Description
A set of algorithms and procedures of a crystal loop centering during X-ray high-throughput protein crystallography experiment has been designed and developed.
A simple algorithm of the crystal loop detection and preliminary recognition has been designed and developed. The crystal loop detection algorithm is based on finding out the crystal loop ending point (opposite to the crystal loop pin) using image cross section (digital image column) profile analysis. The crystal loop preliminary recognition procedure is based on finding out the crystal loop sizes and position using image cross section profile analysis. The crystal loop fine recognition procedure based on Hooke-Jeeves pattern search method with an ellipse as a fitting pattern has been designed and developed. The procedure of restoring missing coordinate of the crystal loop is described. Based on developed algorithms and procedures the optimal autocentering procedure has been designed and developed. A procedure of optimal manual crystal centering (Two Clicks Procedure) is described.
Developed procedures have been integrated into control software system PCCS installed at crystallography beamlines Photon Factory BL5A and PF-AR NW12, KEK.
Primary author
Dr
Yury Gaponov
(Department of atmospheric research, Novosibirsk State University, Novosibirsk, Russia)
Co-authors
Mrs
Kumiko Sasajima
(Structural Biology Research Center, Photon Factory, High Energy Accelerator Research Organization (KEK), Tsukuba, Japan)
Dr
Naohiro Matsugaki
(Structural Biology Research Center, Photon Factory, High Energy Accelerator Research Organization (KEK), Tsukuba, Japan)
Dr
Noriyuki Igarashi
(Structural Biology Research Center, Photon Factory, High Energy Accelerator Research Organization (KEK), Tsukuba, Japan)
Prof.
Soichi Wakatsuki
(Stanford University, Stanford, USA)
