Speaker
Prof.
Sergey Peltek
(Institute of Cytology and Genetics SB RAS (Novosibirsk, Russia))
Description
Mescheryakova I.A. 1, Demidova E.V. 1, Goryachkovskaya T.N. 1, Demidov E.A. 1, Bryanskaya A.V. 1, Sergeeva S.V. 1, Kiselev S.L. 3, Lagarkova M.A. 3, Kulipanov G.N. 2, Semenov A.I.2, Vinokurov N.A.2, Kolchanov N.A.1, Popik V.M.2, Peltek S.E.1
1 The Institute of Cytology and Genetics The Siberian Branch of the Russian Academy of Sciences; 2 Budker Institute of Nuclear Physics the Siberian Branch of the Russian Academy of Sciences; 3 Vavilov Institute of General Genetics, RAS, Moscow,
In connection with the increasingly widespread use of terahertz radiation in the practice and the lack of consensus on the biological effects of terahertz radiation, it seems urgent to study in detail the effects of such exposure as model organisms, including bacteria and human cells.
We studied the impact of terahertz radiation on E. coli biosensor cells containing plasmids with promoters of stress-sensitive genes controlling the expression of GFP. GFP level was measured by fluorometry. The impact of terahertz radiation was nonthermal, i.e. special care was taken to keep specimen temperature in the 35±2 °С range during irradiation so that heat shock genes would not be induced. GFP level was measured by fluorometry.
We found that terahertz radiation activates genes associated with oxidative stress response. Results of Ames test and SOS-chromotest indicate that terahertz radiation doses tested no direct action on DNA mutagenic and does not genotoxic.
Proteomic analysis, it was found that exposure to E. coli cells terahertz radiation leads to increased expression of 14 genes of rapid response. Among these genes discovered glutamine synthetase gene (glnA). Based glnA gene promoter designed biosensor sensitive to the effects of terahertz radiation.
Human embryonic stem cells (hESCs) are extremely sensitive to environmental stimuli, and we therefore utilised this cell model to investigate the non-thermal effects of THz irradiation. We studied DNA damage and transcriptome responses in hESCs exposed to narrow-band THz radiation (2.3 THz) under strict temperature control.
Primary author
Prof.
Sergey Peltek
(Institute of Cytology and Genetics SB RAS (Novosibirsk, Russia))
